Publications

← Back
Journal/Book: Tetrahedron Lett
Published: 1994
Pages: 6649 - 6651
Volume: 35
Issue: 36
Accession no.: 12
Publisher:
ISBN: 0040-4039

Chemical synthesis of 13C-labelled monomers for the solid-phase and template controlled enzymatic synthesis of DNA and RNA oligomers

Quant, S., Wechselberger, R. W., Wolter, M. A., W, Schell, P., J. W. Engels, C. Griesinger, H. Schwalbe
Abstract:
The preparation of 13C-labelled ribonucleosides starting from [13C6]-glucose 1 and the corresponding nucleobases 5a-e or 6a-e (N6-benzoyl-adenine, N2-acetyl-guanine, N4-benzoyl-cytosine, uracil and thymine) in 47 - 66% overall yield is described. Their subsequent transformation into 5'-O-dimethoxytrityl protected DNA-phosphoramidites and 5'-O-dimethoxytrityl-2'-O-trialkylsilyl protected RNA-phosphor-amidites for the solid phase synthesis of DNA- and RNA-oligomers and to 5'-O-ribo- and deoxyribo-nucleosidetriphosphates for template controlled enzymatic synthesis (polymerase- or reverse transcriptase reaction) has been carried out.
Imprint Privacy
© Copyright 2024
Prof. Dr. Harald Schwalbe
Institut für Organische Chemie und Chemische Biologie
Johann Wolfgang Goethe Universität
Max-von-Laue-Str. 7
D-60438 Frankfurt am Main
Website powered by