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Journal/Book: Tetrahedron Lett
Published: 1994
Pages: 6649 - 6651
Volume: 35
Issue: 36
Accession no.: 12
ISBN: 0040-4039

Chemical synthesis of 13C-labelled monomers for the solid-phase and template controlled enzymatic synthesis of DNA and RNA oligomers

Quant, S., Wechselberger, R. W., Wolter, M. A., W, Schell, P., J. W. Engels, C. Griesinger, H. Schwalbe
The preparation of 13C-labelled ribonucleosides starting from [13C6]-glucose 1 and the corresponding nucleobases 5a-e or 6a-e (N6-benzoyl-adenine, N2-acetyl-guanine, N4-benzoyl-cytosine, uracil and thymine) in 47 - 66% overall yield is described. Their subsequent transformation into 5'-O-dimethoxytrityl protected DNA-phosphoramidites and 5'-O-dimethoxytrityl-2'-O-trialkylsilyl protected RNA-phosphor-amidites for the solid phase synthesis of DNA- and RNA-oligomers and to 5'-O-ribo- and deoxyribo-nucleosidetriphosphates for template controlled enzymatic synthesis (polymerase- or reverse transcriptase reaction) has been carried out.
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Prof. Dr. Harald Schwalbe
Institut für Organische Chemie und Chemische Biologie
Johann Wolfgang Goethe Universität
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