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Journal/Book: Journal of Biomolecular NMR
Published: 1994
Pages: 231 - 240
Volume: 4
Issue: 2
Accession no.: 8

Determination of HN,Hα and HN,C′ coupling constants in 13C, 15N-labeled proteins

Weisemann, R., H. Rüterjans, H. Schwalbe, Schleucher, J., W. Bermel, C. Griesinger
Sensitive three-dimensional NMR experiments, based on the E.COSY principle, are presented for the measurement of the 3J(HN,Ha) and 3J(HN,C') coupling constants in uniformly 13C- and 15N-labeled proteins. They employ gradient coherence selection in combination with the sensitivity enhancement method in HSQC-type spectra (Cavanagh et al., 1991; Palmer et al., 1991). In most cases, the two measured coupling constants unambiguously define the ?-angle for protein structure determination. The method is applied to uniformly 13C, 15N-labeled ribonuclease T1.
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Prof. Dr. Harald Schwalbe
Institut für Organische Chemie und Chemische Biologie
Johann Wolfgang Goethe Universität
Max-von-Laue-Str. 7
D-60438 Frankfurt am Main
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